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本產品僅用于科研,不用于臨床
狗腎細胞;Super Tube
上皮細胞
貼壁生長
從表觀正常的成年雌性可卡犬中建立了細胞株MDCK (ATCC CCL-34),狗腎細胞;Super Tube從中克隆建立了狗上皮樣細胞株Super Tube。 當維持高細胞濃度時,Super tube形成大量的小管(據報道,24孔板的每孔鋪7x105細胞,3天內就能形成小管)。 要形成小管,細胞需要每天兩次換液以提供足夠養(yǎng)分。 與原始細胞株相比,Super Tube的c-AMP的檢測水平低得多,在forskolin刺激下讀出的腺苷環(huán)化酶水平也低;它的跨上皮抗性也比Super Dome (ATCC CRL- 2286) 和
DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 胎牛血清,10%
消化3-5分鐘。1:2。3天內可長滿。
不詳
*培養(yǎng)基+8%DMSO
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