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小鼠活化素A酶免試劑盒,(ACV-A)ELISA檢測試劑盒,英文名:ACV-A ELISA Kit,英文縮寫:ACV-A ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠活化素AELISA檢測試劑盒,ACV-A 檢測試劑盒,ACV-A 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠活化素A酶免試劑盒,(ACV-A)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
Slit同源物3(Slit3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 3 (Slit3)48T/96TY13685Rattus norvegicus (Rat)
精氨酰tRNA合成酶(RARS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arginyl tRNA Synthetase (RARS)48T/96TY13686Homo sapiens (Human)
電碳酸氫鈉協(xié)同轉(zhuǎn)運蛋白4(NBC4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4)48T/96TY13687Homo sapiens (Human)
Ras相關(guān)C3肉毒菌毒素底物1(Rac1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)48T/96TY13688Rattus norvegicus (Rat)
脫氧核糖核酸酶Ⅰ(DNASE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Deoxyribonuclease I (DNASE1)48T/96TY13689Bos taurus; Bovine (Cattle)
非受體型蛋白酪氨酸磷酸酶11(PTPN11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11)48T/96TY13690Rattus norvegicus (Rat)
賴氨酰氧化酶樣蛋白1(LOXL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysyl Oxidase Like Protein 1 (LOXL1)48T/96TY13691Homo sapiens (Human)
兒茶酚-O-甲基轉(zhuǎn)移酶(COMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Catechol-O-Methyltransferase (COMT)48T/96TY13692Rattus norvegicus (Rat)
核糖體蛋白S6激酶β1(RPS6Kβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1)48T/96TY13693Homo sapiens (Human)
骨髓基質(zhì)細胞抗原1(BST1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Marrow Stromal Cell Antigen 1 (BST1)48T/96TY13694Homo sapiens (Human)
Saitohin蛋白(STH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Saitohin (STH)48T/96TY13695Homo sapiens (Human)
腺苷A2b受體(ADORA2b)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenosine A2b Receptor (ADORA2b)48T/96TY13696Homo sapiens (Human)
鳥苷酸環(huán)化酶激活因子2B(GUCA2B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Guanylate Cyclase Activator 2B (GUCA2B)48T/96TY13697Homo sapiens (Human)
組織金屬蛋白酶抑制因子1(TIMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY13698Bos taurus; Bovine (Cattle)
饑餓素(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ghrelin (GHRL)48T/96TY13699Ovis aries; Ovine (Sheep)
生長分化因子11(GDF11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 11 (GDF11)48T/96TY13700Mus musculus (Mouse)
SPARC樣蛋白1(SPARCL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for SPARC Like Protein 1 (SPARCL1)48T/96TY13701Rattus norvegicus (Rat)
防御素β112(DEFβ112)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Defensin Beta 112 (DEFb112)48T/96TY13702Homo sapiens (Human)
血管生成素樣蛋白6(ANGPTL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 6 (ANGPTL6)48T/96TY13703Rattus norvegicus (Rat)
IgG-Fc片段低親和力受體Ⅲa(FcγR3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A)48T/96TY13704Homo sapiens (Human)
信號傳導(dǎo)轉(zhuǎn)錄激活因子3(STAT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 3 (STAT3)48T/96TY13705Rattus norvegicus (Rat)
載脂蛋白C3(APOC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein C3 (APOC3)48T/96TY13706Rattus norvegicus (Rat)
載脂蛋白C2(APOC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein C2 (APOC2)48T/96TY13707Rattus norvegicus (Rat)
Janus激酶2(JAK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Janus Kinase 2 (JAK2)48T/96TY13708Rattus norvegicus (Rat)
旁血小板溶蛋白(PPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Periplakin (PPL)48T/96TY13709Homo sapiens (Human)
Engrailed同源框蛋白2(EN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Engrailed Homeobox Protein 2 (EN2)48T/96TY13710Homo sapiens (Human)
膠質(zhì)細胞系來源神經(jīng)營養(yǎng)因子受體α1(GFRα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 (GFRa1)48T/96TY13711Homo sapiens (Human)
腫瘤壞死因子α誘導(dǎo)蛋白6(TNFαIP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)48T/96TY13712Mus musculus (Mouse)
多聚A特異性核糖核酸酶(PARN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Poly A Specific Ribonuclease (PARN)48T/96TY13713Homo sapiens (Human)
氯化物細胞內(nèi)通道蛋白1(CLIC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chloride Intracellular Channel Protein 1 (CLIC1)48T/96TY13714Homo sapiens (Human)
醌型二氫蝶啶還原酶(QDPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Quinoid Dihydropteridine Reductase (QDPR)48T/96TY13715Homo sapiens (Human)
肌酸轉(zhuǎn)運蛋白(CRT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Creatine Transporter (CRT)48T/96TY13716Homo sapiens (Human)
蛋氨酸腺苷轉(zhuǎn)移酶Ⅰα(MAT1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methionine Adenosyltransferase I Alpha (MAT1a)48T/96TY13717Mus musculus (Mouse)
雙氧化酶2(DUOX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dual Oxidase 2 (DUOX2)48T/96TY13718Homo sapiens (Human)
雙氧化酶2(DUOX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dual Oxidase 2 (DUOX2)48T/96TY13719Rattus norvegicus (Rat)
組蛋白H1(H1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histone H1 (H1)48T/96TY13720Homo sapiens (Human)
苯丙氨酸羥化酶(PAH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phenylalanine Hydroxylase (PAH)48T/96TY13721Homo sapiens (Human)
蘇氨酰tRNA合成酶(TARS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Threonyl tRNA Synthetase (TARS)48T/96TY13722Homo sapiens (Human)
γ-谷氨酰水解酶(γGH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gamma-Glutamyl Hydrolase (gGH)48T/96TY13723Homo sapiens (Human)
胃泌酸調(diào)節(jié)素(OXM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxyntomodulin (OXM)48T/96TY13724Homo sapiens (Human)
硒結(jié)合蛋白1(SELENBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selenium Binding Protein 1 (SELENBP1)48T/96TY13725Homo sapiens (Human)
細胞色素P450家族成員2E1(CYP2E1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 2E1 (CYP2E1)48T/96TY13726Mus musculus (Mouse)
衰老關(guān)鍵蛋白2(FBLN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibulin 2 (FBLN2)48T/96TY13727Homo sapiens (Human)
單核細胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY13728Sus scrofa; Porcine (Pig)
趨化因子C-X-C-基元受體5(CXCR5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Receptor 5 (CXCR5)48T/96TY13729Homo sapiens (Human)
白介素13受體α2(IL13Rα2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 13 Receptor Alpha 2 (IL13Ra2)48T/96TY13730Mus musculus (Mouse)
層黏連蛋白α3(LAMα3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Laminin Alpha 3 (LAMa3)48T/96TY13731Homo sapiens (Human)
亮氨酸豐富重復(fù)激酶2(LRRK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leucine Rich Repeat Kinase 2 (LRRK2)48T/96TY13732Homo sapiens (Human)
甘露糖受體C2(MRC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Receptor C Type 2 (MRC2)48T/96TY13733Homo sapiens (Human)
E1A激活基因阻遏子1(CREG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cellular Repressor Of E1A Stimulated Genes 1 (CREG1)48T/96TY13734Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。