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小鼠磷酸化細(xì)胞外信號調(diào)節(jié)激酶酶免試劑盒,(pERK)ELISA檢測試劑盒 ,英文名:pERK ELISA Kit ,英文縮寫:pERK ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠磷酸化細(xì)胞外信號調(diào)節(jié)激酶ELISA檢測試劑盒,pERK 檢測試劑盒, pERK 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠磷酸化細(xì)胞外信號調(diào)節(jié)激酶酶免試劑盒,(pERK)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
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早期B-細(xì)胞因子3(EBF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Early B-Cell Factor 3 (EBF3)96TY17308Rattus norvegicus (Rat)
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成纖維生長因子受體底物3(FRS3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor Receptor Substrate 3 (FRS3)96TY17312Homo sapiens (Human)
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成纖維生長因子受體底物3(FRS3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor Receptor Substrate 3 (FRS3)96TY17314Rattus norvegicus (Rat)
白介素1ζ(IL1ζ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Zeta (IL1z)96TY17315Homo sapiens (Human)
白介素1ε(IL1ε)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Epsilon (IL1e)96TY17316Homo sapiens (Human)
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生長因子受體結(jié)合蛋白7(Grb7)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Growth Factor Receptor Bound Protein 7 (Grb7)96TY17325Homo sapiens (Human)
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成纖維細(xì)胞生長因子結(jié)合蛋白2(FGFBP2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor Binding Protein 2 (FGFBP2)96TY17341Homo sapiens (Human)
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各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。